The FRAP assay of Benzie and Strain [13] was used with minor modifications that allowed quantification of most water- and fat-soluble antioxidants [16,17]. A Technicon RA 1000 system (Technicon instruments corporation, New York, USA) was used for the measurements of absorption changes that appear when the TPTZ-Fe3+ complex reduces to the TPTZ-Fe2+ form in the presence of antioxidants. An intense blue color with absorption maximum at 593 nm develops. The measurements were performed at 600 nm after 4 min incubation. An aqueous solution of 500 μmol/L FeSO4 × 7 H2O was used for calibration of the instrument. Validation of the assay is described in Halvorsen et al. 2002 [17]. Briefly, the within-day repeatability measured as relative standard deviation (RSD) in standard solutions ranged from 0.4% to 6%. The between-day repeatability was < 3%. The variation in the values for replicate food items obtained from the same source were typically between 3 and 10 RSD%.
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Sugar addiction is a real and growing concern for a large majority of the world’s population. But how exactly does this happen? The Huffington Post explains that when a person consumes sugar, the tongue’s taste buds become activated and send signals to the brain, “lighting up reward pathways and causing a surge of feel-good hormones, like dopamine, to be released.”
The frequent consumption of small quantities of dark chocolate is linked to lower BMI, according to a study published in the Journal of Internal Medicine. Chocolate consumption frequency (via a questionnaire) and BMI (weight divided by height in meters squared) were analyzed among 1,018 men and women aged 20 to 85. Mood, activity per 7-day period, fruit and vegetable intake and saturated fat intake were considered and factored into the researchers analysis as well. All in all, the correlation between chocolate consumption and low BMI upheld. The mean age of subjects was 57, of which 68 percent were male, with a BMI of 28 who ate dark chocolate two times per week and exercised about 3.5 times per week. 
Prior says that the data should prove useful for consumers seeking to include more antioxidants in their diet. But he cautions that total antioxidant capacity of the foods does not necessarily reflect their potential health benefit, which depends on how they are absorbed and utilized in the body. Researchers are still trying to better understand this process, he adds.
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Interestingly, the antioxidant content in human breast milk is comparable to that in pomegranate juice, strawberries and coffee and on average higher than the antioxidant content observed in the commercially available infant formulas analyzed in our study. Breakfast cereals are also potential important sources of antioxidants; some of these products have antioxidant contents comparable to berries, which are fairly high, compared to other grain products and may be due to antioxidants added to the products in fortification process.
To date, studies have confirmed that chronic inflammation contribute to factors that increase your risk of developing cancer, including DNA mutations and cancer cell growth. Research has confirmed that the antioxidants in dark chocolate have a strong ability to fight the DNA damage that could lead to cancer development, as well as reduce certain inflammation enzymes that could encourage its growth (12).
Important Disclaimer: The information contained on Health Ambition is intended for informational and educational purposes only. Any statements made on this website have not been evaluated by the FDA and any information or products discussed are not intended to diagnose, cure, treat or prevent any disease or illness. Please consult a healthcare practitioner before making changes to your diet or taking supplements that may interfere with medications.
The two opposing extracts were essentially left in vivo (outside of the human body) to battle each other. The resulting statistics show that chocolate's antioxidants (at least, in vivo) are extremely effective at reducing free radicals. While they may behave differently in the body, relevant studies also show that chocolate is effective at battling free radicals in vitro.

Back when food was way scarcer, our ancient ancestors needed to take every advantage they had to consume high calorie foods. So the human brain evolved to perceive sugar—and fat—as very rewarding, says Schwartz. Today, our brains are still wired for feast or famine, even though you can buy thousands of calories of food for a couple bucks at the local convenience store.  

Certain vitamins and minerals support healthy blood sugar levels. Magnesium in leafy green vegetables and nuts, for instance, can improve insulin sensitivity. Eating a whole, unprocessed foods diet can provide these nutrients to optimize immune function. A multivitamin-mineral (available for men, women, and kids) can cover the nutrient bases you might not be getting from food.

People with high levels of sugar consumption are much more likely to become obese or overweight, no matter what age — even children. In fact, children who drink a serving of sugar-sweetened beverage daily have a 60 percent greater chance of become obese. This sets your child up for a lifetime of being overweight or obese — plus, all of the potential health


- Cut one source of sugar each week. If the thought of drastically reducing your sugar intake makes you nervous, take a gradual approach. Start cutting sugary treats a little at a time. Cut dessert to one or two days a week. Stop adding sugar to your coffee. Skip the weekly office donut tray. This gives you a chance to adjust to less sugar over time.
Plants are humanity's greatest ally in the fight against climate change. Plants soak up carbon dioxide and turn it into leaves and branches. The more trees humans plant, the less heat-trapping carbon pollution in the air. Unfortunately, plants require a lot of water and land, so much that humans might need a new to find a new ally to help draw down all that carbon.
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